Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE EPS8 antibody diluted at 1:1000Application
EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EPS8 antibody [C3], C-term dilution: 1:500.Application
Immunoprecipitation of EPS8 protein from HepG2 whole cell extracts using 5 μg of EPS8 antibody [C3], C-term . Western blot analysis was performed using EPS8 antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | EPS8 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to EPS8 (epidermal growth factor receptor pathway substrate 8)This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EPS8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rhesus MonkeyMouse (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPS8 antibody epidermal growth factor receptor kinase substrate 8 antibody epidermal growth factor receptor pathway substrate 8 antibody |
|---|---|
| Target | EPS8 |
| Molecular Weight(MW) | 92 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7.5% SDS PAGE EPS8 antibody diluted at 1:1000
Application
EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EPS8 antibody [C3], C-term dilution: 1:500.
Application
Immunoprecipitation of EPS8 protein from HepG2 whole cell extracts using 5 μg of EPS8 antibody [C3], C-term . Western blot analysis was performed using EPS8 antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
