Application
Sample(30 μg of whole cell lysate) A:HeLa S3 B:MOLT4 15% SDS PAGE diluted at 1:1000Application
ERAB antibody detects ERAB protein at mitochondria on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. ERAB antibody dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ERAB antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Neuro 2A B: GL261 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: PC-12 12% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ERAB antibody at 1:100 dilution.Application
ERAB antibody detects ERAB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ERAB antibody dilution: 1:500.Application
ERAB antibody detects ERAB protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ERAB antibody dilution: 1:500.| Product Name | ERAB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERAB (hydroxysteroid (17-beta) dehydrogenase 10)This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERAB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Rhesus Monkey, BovineZebrafish (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 17b-HSD10 antibody ABAD antibody CAMR antibody DUPXp11.22 antibody ERAB antibody HADH2 antibody HCD2 antibody MHBD antibody MRPP2 antibody MRX17 antibody MRX31 antibody MRXS10 antibody SCHAD antibody SDR5C1 antibody HSD17B10 antibody endopla |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Sample(30 μg of whole cell lysate) A:HeLa S3 B:MOLT4 15% SDS PAGE diluted at 1:1000
Application
ERAB antibody detects ERAB protein at mitochondria on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. ERAB antibody dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ERAB antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Neuro 2A B: GL261 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: PC-12 12% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ERAB antibody at 1:100 dilution.
Application
ERAB antibody detects ERAB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ERAB antibody dilution: 1:500.
Application
ERAB antibody detects ERAB protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ERAB antibody dilution: 1:500.| Positive Control | HeLaS3 , Molt-4 , Neuro2A , GL261 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
