Application
Human Breast Carcinoma stained with anti-ERK-1 antibody| Product Name | ERK1 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ERK1 predilutedERK1 is ubiquitously expressed in various tissues and cells. It is activated by phosphorylation of neighbouring serine and threonine residues by MEK1 and MEK2 in response to diverse stimuli such as growth factors and mitogens. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Rat) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Use at a dilution of 1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p44 PRKM3 601795 ERK1 Mapk3 p44erk1 50689 p44mapk 5595 Erk-1 P27361 Esrk1 Erk 1 |
|---|---|
| Target | ERK1 |
| Cellular Localization | Cytoplasmic |
Application
Human Breast Carcinoma stained with anti-ERK-1 antibody| Positive Control | A431 cells, Breast |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
