Application
Mouse Mapk3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody at 1:1000 at each lane.Application
Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Product Name | Mapk3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1, Mapk3, Erk1, Prkm3 |
| Product description | Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) and heat shock factor protein 4 (HSF4) (By similarity). Phosphorylates SPZ1.1) Chen, W., et al. Biochem. Biophys. Res. Commun. 401(3):339-343(2010) |
| Immunogen | This Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of mouse Mapk3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Mapk3 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase 3 MAP kinase 3 MAPK 3 ERT2 Extracellular signal-regulated kinase 1 ERK-1 Insulin-stimulated MAP2 kinase MAP kinase isoform p44 p44-MAPK MNK1 Microtubule-associated protein 2 kinase p44-ERK1 Mapk3 Erk1 Prkm3 |
| Molecular Weight(MW) | 43 kDa |
Application
Mouse Mapk3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody at 1:1000 at each lane.
Application
Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
