Application
Western blot analysis of extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase(Phospho-Tyr204) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .IHC
A, Hematoxylin and eosin staining (scale bar: 20 μm). B, Masson's trichrome staining (scale bar: 20 μm). C–F, Immunohistochemical staining to detect Hsp70 (C, scale bar: 20 μm), p-Akt (D, scale bar: 20 μm), p-JNK (E, scale bar: 20 μm), and p-ERK (F, scale bar: 20 μm) expression.WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium. Previous| Product Name | ERK1/2 (phospho Tyr204) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ERK1/2 (phospho Tyr204)Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase. |
| Modification | p-Tyr204 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | ERK1 HS44KDAP HUMKER1A MGC20180 P44ERK1 P44MAPK PRKM3 MAPK3 extracellular signal-regulated kinase 1 MAPK 1 microtubule-associated protein 2 kinase MAP kinase 3 mitogen-activated protein kinase 3 MAP kinase 1 mitogen-activated protein kinase 1 |
|---|---|
| Target | ERK1/2 (phospho Tyr204) |
| Molecular Weight(MW) | 42, 44 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase(Phospho-Tyr204) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .
IHC
A, Hematoxylin and eosin staining (scale bar: 20 μm). B, Masson's trichrome staining (scale bar: 20 μm). C–F, Immunohistochemical staining to detect Hsp70 (C, scale bar: 20 μm), p-Akt (D, scale bar: 20 μm), p-JNK (E, scale bar: 20 μm), and p-ERK (F, scale bar: 20 μm) expression.
WB
A–E, Western blotting evaluations of Hsp70 (A), cleaved caspase-3 (B), p-Akt and Akt (C), p-JNK and JNK (D), and p-ERK and ERK (E) levels in the myocardium. Previous| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
