Rabbit polyclonal antibody to ERK1/2Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Immunogen
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
Rat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
MGC20180 mitogenactivated protein kinase3 mitogenactivated protein kinase 3 HUMKER1A PRKM3 ERK1 mitogen-activated protein kinase 3 MAPK3 P44MAPK HS44KDAP P44ERK1 MAPK1/2
Target
ERK1/2
Molecular Weight(MW)
42/44 (note)
Application
Application
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~42-44 ERK/MAPK protein using ERK/MAPK antibody .
Application Notes
Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles.
Storage Buffer
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.