Application
Extracts of PC12 cells unstimulated (-) or stimulated with 20 ng/ml NGF for 20 minutes (+) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4 degree C, then incubated with 0.5 ug/ml ERK1&2 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that the ERK1&2 antibody allows the total amount of ERK1&2 to be measured (non-phoshphorylated as well as phosphorylated). The resulting signals serve as useful controls in assessing the degree of up-regulation as detected using an antibody to the dually phosphorylated (active) form of ERK1&2| Product Name | ERK1/2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ERK1/2ERK (extracellular signal regulated kinase), also known as MAPK (mitogen-activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. |
| Immunogen | Synthetic peptide, corresponding to C terminal amino acids 317-339 of Human ERK1 + ERK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1/1000. Predicted molecular weight: ~42-44 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERK1 & ERK2 176982 PKC2 116590 Erk2 5590 Q05513 Mapk1 PRKCZ |
|---|---|
| Target | ERK1/2 |
| Cellular Localization | Cell Membrane |
Application
Extracts of PC12 cells unstimulated (-) or stimulated with 20 ng/ml NGF for 20 minutes (+) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4 degree C, then incubated with 0.5 ug/ml ERK1&2 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that the ERK1&2 antibody allows the total amount of ERK1&2 to be measured (non-phoshphorylated as well as phosphorylated). The resulting signals serve as useful controls in assessing the degree of up-regulation as detected using an antibody to the dually phosphorylated (active) form of ERK1&2| Positive Control | A431, PC12, and NIH3T3 cells |
|---|---|
| Application Notes | WB: 1/1000. Predicted molecular weight: ~42-44 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide. Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
