Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42k-44k ERK/MAPK protein doublet.| Product Name | ERK1 / 2 (phospho Thr202 / Tyr204) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Erk2, Erk2, Mapk, Prkm1, ERT1, MAP kinase 1 |
| Product description | Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.1) Tanoue, T.J. and Nishida, E., "Molecular recognitions in the MAP kinase cascades. Cellular Signaling 15 (2003) 455 - 462. |
| Immunogen | ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK. |
| Modification | p-Thr202 / Tyr204 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
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| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Mapk1 |
|---|---|
| Alternative Names | Erk2 Erk2 Mapk Prkm1 ERT1 MAP kinase 1 |
| Molecular Weight(MW) | 42/44 |
| Tissue Specificity | ERK1/2 antibody is specific for the 42/44 k ERK/MAPK protein phosphorylated at Thr202 and Tyr204.. |
Application
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42k-44k ERK/MAPK protein doublet.| Application Notes | Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚C. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
