Application
Western blot analysis of p44/42 MAPK using rat kidney tissue lysate (Lane 1), mouse 3T3 cell lysate (Lane 2) and Jurkat cell lysate (Lane 3 & 4).| Product Name | ERK1/2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ERK1/2The extracellular signal-regulated kinase 1 and 2 (Erk1 and Erk2) are closely related mitogen activated protein (MAP) kinases that are activated through the extracellular stimulation by many growth factors, mitogens and differentiation promoting agents via a protein kinase cascade. Erk1/2 are activated approximately 1000 fold by phosphorylation of neighboring threonine and tyrosine residues by Mek1 and Mek2. Both sites must be phosphorylated for maximum activity. Erk1/2 kinases are ubiquitously distributed in the nervous system, as well as a broad range of cells and tissues. These two kinases represent proximal kinases in the classical MAP kinase cascade pathway which links growth and differentiation signals at the cell surface (through tyrosine kinase) with transcription in the nucleus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot analysis (1-4 µg/ml). The optimal conditions should be determined individually.: |
|
| Species Reactivity | HumanMouseChickenRatSheep |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKM2 MAPK2 p42-MAPK p38 ERK P42MAPK ERK2 p40 PRKM1 p41 ERK-2 ERT1 MAPK1 p41mapk ERK 2 p42 MAPK |
|---|---|
| Target | ERK1/2 |
| Tissue Specificity | The antibody recognizes 42 kDa and 44 kDa of Erk1/Erk2 (p44/p42 MAP kinase). |
Application
Western blot analysis of p44/42 MAPK using rat kidney tissue lysate (Lane 1), mouse 3T3 cell lysate (Lane 2) and Jurkat cell lysate (Lane 3 & 4).| Application Notes | Western Blot analysis (1-4 µg/ml). The optimal conditions should be determined individually.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20ºC. Avoid multiple freeze-thaw cycles.The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal |
