Application
ApplicationApplication
Human Breast Carcinoma stained with anti-ERK-2 antibodyApplication
staining ERK2 in breast carcinoma by IHC-P| Product Name | ERK2 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ERK2 predilutedERK2 is activated by MEK2, which phosphorylates neighbouring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Mouse) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| IHC-P: Use at a dilution of 1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 116590 ERK2 Mapk1 |
|---|---|
| Target | ERK2 |
| Cellular Localization | Cytoplasmic |
Application
Application
Application
Human Breast Carcinoma stained with anti-ERK-2 antibody
Application
staining ERK2 in breast carcinoma by IHC-P| Positive Control | A431 cells, Breast carcinoma |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
