Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ERM antibody detects ERM protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ERM antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ERM antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERM antibody at 1:500 dilution.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ERM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERM (ets variant 5)Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, BovineRat (95%), Pig (98%), Chicken (88%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERM antibody ETV5 antibody ets-related molecule antibody ets-related protein ERM antibody ETS translocation variant 5 antibody ets variant 5 antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ERM antibody detects ERM protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ERM antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ERM antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERM antibody at 1:500 dilution.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Raji , NCI-H929 , mouse testis |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
