Application
Immunofluorescence analysis of methanol-fixed Hep3B, using ERO1L antibody at 1:500 dilution.Application
ERO1L antibody detects ERO1L protein by Western blot analysis. A. 30 µg HCT116 whole cell lysate/extract B. 30 µg MCF-7 whole cell lysate/extract 10 % SDS-PAGE ERO1L antibody dilution: 1:1000Application
ERO1L antibody detects ERO1L protein by Western blot analysis. A. 50 µg mouse stomach lysate/extract 10 % SDS-PAGE ERO1L antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ERO1L antibody at 1:100 dilution.| Product Name | ERO1L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERO1L (ERO1-like (S. cerevisiae))Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERO1L. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (90%), Pig (96%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERO1-alpha antibody ERO1A antibody ERO1L antibody ERO1-like protein alpha antibody oxidoreductin-1-L-alpha antibody ERO1-L antibody endoplasmic oxidoreductin-1-like protein antibody ERO1-L-alpha antibody ERO1-like (S. cerevisiae) antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side |
Application
Immunofluorescence analysis of methanol-fixed Hep3B, using ERO1L antibody at 1:500 dilution.
Application
ERO1L antibody detects ERO1L protein by Western blot analysis. A. 30 µg HCT116 whole cell lysate/extract B. 30 µg MCF-7 whole cell lysate/extract 10 % SDS-PAGE ERO1L antibody dilution: 1:1000
Application
ERO1L antibody detects ERO1L protein by Western blot analysis. A. 50 µg mouse stomach lysate/extract 10 % SDS-PAGE ERO1L antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ERO1L antibody at 1:100 dilution.| Positive Control | HCT116 , MCF-7 , mouse stomach |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
