Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ERP29 antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ERP29 antibody detects ERP29 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. ERP29 antibody dilution: 1:500.Application
ERP29 antibody detects ERP29 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ERP29 antibody dilution: 1:500.Application
ERP29 antibody detects ERP29 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. ERP29 antibody dilution: 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ERP29 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | ERP29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ERP29 (endoplasmic reticulum protein 29)This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERP29. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Sheep, Rhesus Monkey, BovineChicken (83%), Sheep (95%), Rhesus Monkey (99%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C12orf8 antibody ERp28 antibody ERp31 antibody PDI-DB antibody PDIA9 antibody ERP29 antibody endoplasmic reticulum lumenal protein ERp28 antibody endoplasmic reticulum resident protein 28 antibody endoplasmic reticulum resident protein 29 antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Endoplasmic reticulum lumen , Melanosome |
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ERP29 antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ERP29 antibody detects ERP29 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. ERP29 antibody dilution: 1:500.
Application
ERP29 antibody detects ERP29 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ERP29 antibody dilution: 1:500.
Application
ERP29 antibody detects ERP29 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. ERP29 antibody dilution: 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ERP29 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
