Application
Western Blot analysis (reducing conditions) of recombinant protein EsaT-6 using polyclonal anti-EsaT-6 antibody . Lane 1, 3, 5: cell lysates of non-transfected E. coli (negative controls) Lane 2, 4, 6: cell lysate of EsaT-6-transfected E. coli; dilution (2) 1 μg/ml, (4) 0.2 μg/ml,(6) 0.1 μg/ml; detection with Donkey anti-rabbit / HRP secondary antibody| Product Name | ESAT6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ESAT6The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. |
| Immunogen | EsaT-6 (Rv3875) of Mycobacterium tuberculosis |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500 to 1 1:2,000. Optimal dilutions/concentrations should be determined by the end use.: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Target | ESAT6 |
|---|---|
| Tissue Specificity | The polyclonal antibody reacts with EsaT-6, a 11,8 kDa antigen encoded by gene Rv3875 of Mycobacterium tuberculosis.The antibody recognizes EsaT-6 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli. |
Application
Western Blot analysis (reducing conditions) of recombinant protein EsaT-6 using polyclonal anti-EsaT-6 antibody . Lane 1, 3, 5: cell lysates of non-transfected E. coli (negative controls) Lane 2, 4, 6: cell lysate of EsaT-6-transfected E. coli; dilution (2) 1 μg/ml, (4) 0.2 μg/ml,(6) 0.1 μg/ml; detection with Donkey anti-rabbit / HRP secondary antibody| Application Notes | For WB: Use at a dilution of 1:500 to 1 1:2,000. Optimal dilutions/concentrations should be determined by the end use.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, 0.09% sodium azide. |
