Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using ESD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
ESD antibody detects ESD protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ESD antibody diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE FGH antibody diluted at 1:1000| Product Name | ESD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ESD (esterase D)This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ESD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineZebrafish (83%), Xenopus laevis (82%), Pig (97%), Chicken (85%), Rhesus Monkey (97%), Bovine (89%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FGH antibody FLJ11763 antibody ESD antibody esterase 10 antibody methylumbelliferyl-acetate deacetylase antibody esterase D/formylglutathione hydrolase antibody S-formylglutathione hydrolase antibody esterase D antibody |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cytoplasmic vesicle |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using ESD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
ESD antibody detects ESD protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ESD antibody diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE FGH antibody diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
