Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: MCF7 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Whole cell extract (60 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate .| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1F3] to Estrogen Receptor alphaThe Estrogen Receptor (ER) is a 65a protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins. |
| Immunogen | The region encoding amino acids 1-190 expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F3 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P03372 ESR major ORF Era ER 133430 ER alpha DKFZp686N23123 ESRA NR3A1 |
|---|---|
| Target | ER alpha |
| Molecular Weight(MW) | 65 kDa (note) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: MCF7 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (60 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate .| Positive Control | MCF7 , MCF7 , ESR1-transfected 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
