Application
Western blot analysis using ESR1 mouse mAb against MCF-7 (1), T47D (2) and SKBR3 (3) cell lysate.Application
Immunofluorescence analysis of Hela cells using ESR1 mouse mAb ( green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidinApplication
Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using ESR1 mouse mAb with DAB staining.| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6B6] to ESR1This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human ESR1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6B6 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ESRA ESR1 NR3A1 ESR ER ERA |
|---|---|
| Target | ESR1 |
| Molecular Weight(MW) | 66 kDa (note) |
Application
Western blot analysis using ESR1 mouse mAb against MCF-7 (1), T47D (2) and SKBR3 (3) cell lysate.
Application
Immunofluorescence analysis of Hela cells using ESR1 mouse mAb ( green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
Application
Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using ESR1 mouse mAb with DAB staining.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
