Application
Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor antibody.| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Estrogen ReceptorThis antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. |
| Immunogen | Prokaryotic recombinant protein corresponding to the full-length estrogen receptor molecule |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F11 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| This antibody may be diluted to a titer of 1:20 to 1:80 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P03372 ESR major ORF Era ER NR3A1 ESRA DKFZp686N23123 133430 ESR1 ER alpha 2099 |
|---|---|
| Target | ER alpha |
| Cellular Localization | Nuclear |
Application
Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor antibody.| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:20 to 1:80 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Can also be used for: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100): |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
