Application
IHC-P analysis of human breast cancer tissue using Estrogen Receptor alpha antibody. Antigen retrieval using citrate buffer at pH6.2.Application
WB analysis of MCF-7 cell lysate using Estrogen Receptor alpha antibody [6F11].| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6F11] to Estrogen Receptor alphaThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human estrogen receptor (alpha form) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F11 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| Western Blotting: 1/50 - 1/100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Era ESR ER NR3A1 ESRA estrogen receptor 1 ESR1 |
|---|---|
| Target | Estrogen Receptor alpha |
| Tissue Specificity | This antibody recognizes the human estrogen receptor alpha chain. |
Application
IHC-P analysis of human breast cancer tissue using Estrogen Receptor alpha antibody. Antigen retrieval using citrate buffer at pH6.2.
Application
WB analysis of MCF-7 cell lysate using Estrogen Receptor alpha antibody [6F11].| Application Notes | Western Blotting: 1/50 - 1/100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
