Application
IHC-P analysis of human breast carcinoma using Estrogen Receptor alpha antibody [6F11].Application
Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor antibody (cat. # ).| Product Name | Estrogen Receptor alpha antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [6F11] to Estrogen Receptor (ready-to-use)The estrogen receptor (ER) is a 66 kDa protein that mediates the actions of estrogens in estrogen responsive tissues. It is a member of a large superfamily of nuclear hormone receptors that function as ligand activated transcription factors. The ER gene consists of more than 140 kb of genomic DNA divided into 8 exons. These translate into a protein with six functionally discrete domains, labeled A through F. A second form of the estrogen receptor, ER beta has recently been described. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti estrogen (e.g. tamoxifen) therapy. |
| Immunogen | Prokaryotic recombinant protein corresponding to the full-length estrogen receptor molecule |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F11 |
| Host Species | Mouse |
| Tested Applications | AFIHC-P |
| For IHC-P: Ready to use - Incubate for 30 minutes. High temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 is recommended. For AF: Ready to use - Incubate for 30 minutes. No further titration is required. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P03372 ESR major ORF Era ER NR3A1 ESRA DKFZp686N23123 133430 ESR1 ER alpha 2099 |
|---|---|
| Target | ER alpha |
| Tissue Specificity | Specific to the estrogen receptor and recognizes full-length polypeptide chain of the receptor. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. |
| Cellular Localization | Nuclear |
Application
IHC-P analysis of human breast carcinoma using Estrogen Receptor alpha antibody [6F11].
Application
Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor antibody (cat. # ).| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | For IHC-P: Ready to use - Incubate for 30 minutes. High temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 is recommended. For AF: Ready to use - Incubate for 30 minutes. No further titration is required. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | TCS containing 0.09% sodium azide |
