Application
WB analysis of 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein using ER alpha antibody at a concentration of 7 μg/ml.Application
ICC analysis of COS-7 cells transiently overexpressing human ERα or ERs1 using ER alpha antibody at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin.| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [GT9077] to Estrogen Receptor alphaThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008] |
| Immunogen | Monoclonal antibody raised in mouse against the NH2 terminus of the human ERα (estrogen receptor alpha), using a KLH-conjugated synthetic peptide (Q19-K32). |
| Clonality | Monoclonal |
|---|---|
| Clone Number | GT9077 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWBChIP |
| ChIP (5 µg/IP), Western blotting (7 µg/ml), Immunochemistry (15 µg/ml): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Era ESR ER NR3A1 ESRA estrogen receptor 1 ESR1 |
|---|---|
| Target | Estrogen Receptor alpha |
Application
WB analysis of 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein using ER alpha antibody at a concentration of 7 μg/ml.
Application
ICC analysis of COS-7 cells transiently overexpressing human ERα or ERs1 using ER alpha antibody at a concentration of 15 μg/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin.| Application Notes | ChIP (5 µg/IP), Western blotting (7 µg/ml), Immunochemistry (15 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide; purified by ammonium sulphate precipitation followed by dialysis. |
