Application
Western blot analysis of ESR1: Lane 1: MWM Lane 2: Estrogen Receptor 1 GST-tagged ER1 protein control runs at 92 kDa| Product Name | Estrogen Receptor alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [h-151] to Estrogen Receptor alphaThis antibody recognizes human ER-alpha, a 65 kD protein and a member of the steroid family of nuclear receptors. ER-alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, ER-alpha can positively or negatively regulate gene transcription. |
| Immunogen | Synthetic peptide: RAANLWPSPLMIKR conjugated to KLH, corresponding to amino acids 287-300 of Human Estrogen Receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | h-151 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF: Use at an assay dependent dilution. IP: Use at a concentration of 15 μg/ml. WB: 1:250. Predicted molecular weight: 71.4 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | P03372 ESR major ORF Era ER NR3A1 ESRA DKFZp686N23123 133430 ESR1 ER alpha 2099 |
|---|---|
| Target | Estrogen Receptor alpha |
| Tissue Specificity | Binding specificity was confirmed in peptide inhibition studies with the intact protein. The antibody does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor. In immunoprecipitation studies, this antibody has been shown to only IP the active receptor. |
| Cellular Localization | Nuclear |
Application
Western blot analysis of ESR1: Lane 1: MWM Lane 2: Estrogen Receptor 1 GST-tagged ER1 protein control runs at 92 kDa| Application Notes | ICC/IF: Use at an assay dependent dilution. IP: Use at a concentration of 15 μg/ml. WB: 1:250. Predicted molecular weight: 71.4 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
