Rabbit polyclonal antibody to Estrogen SulfotransferaseMammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3’-phosphate-5’-phosphosulfate. Sulfurylated steroids do not bind to the estrogen receptor with high affinity and, therefore, are hormonally inactive. EST plays an important role in controlling the intracellular level of the receptor-active estrogens.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. Detects 31 kDa Estrogen Sulfotransferase (EST) of human and rat origins.:
Species Reactivity
HumanRat
Concentration
0.5mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
STE SULT1E1 MGC34459 EST1 EST EST-1 EST 1
Target
Estrogen Sulfotransferase
Tissue Specificity
This antibody is specific for Estrogen Sulfotransferase.
Application
Application
Western blot analysis of Estrogen Sulfotransferase (EST) with Jurkat cell lysates (Lane 1, 2).
Application Notes
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. Detects 31 kDa Estrogen Sulfotransferase (EST) of human and rat origins.:
Additional Information
Form
Liquid
Storage Instructions
The antibody solution should be gently mixed before use. Stored at -20 °C for future use.