Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000| Product Name | ET-3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Endothelin 3 (endothelin 3)The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ET-3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ET3 antibody HSCR4 antibody MGC15067 antibody MGC61498 antibody WS4B antibody EDN3 antibody endothelin-3 antibody preproendothelin-3 antibody endothelin 3 antibody |
|---|---|
| Target | Endothelin 3 |
| Molecular Weight(MW) | 25 kDa (note) (5) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
