Application
ChIP was performed with SKNO-1 nuclear extract and either 4 μl of ETO antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to H2B, OGG1, FUT7, NFE2 or VEGF.Application
ELISA was performed using a serial dilution of ETO / RUNX1T1 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,300.| Product Name | ETO / RUNX1T1 antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ETO / RUNX1T1 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAChIP |
| ChIP/ChIP-seq (1 μg/ChIP), ELISA (1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ZMYND2 AML1T1 RUNX1T1 CBFA2T1 CDR MTG8 runt-related transcription factor 1 translocated to 1 (cyclin D-related) |
|---|---|
| Target | ETO |
Application
ChIP was performed with SKNO-1 nuclear extract and either 4 μl of ETO antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to H2B, OGG1, FUT7, NFE2 or VEGF.
Application
ELISA was performed using a serial dilution of ETO / RUNX1T1 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,300.| Application Notes | ChIP/ChIP-seq (1 μg/ChIP), ELISA (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
