Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in WB on Human 721_B at 1 ug/ml.| Product Name | RUNX1T1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUNX1T1, CDR, ETO, MTG8, AML1T1, ZMYND2, CBFA2T1 |
| Product description | RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t (8;21) (q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.1) Lausen, J., et al., (2004) J. Biol. Chem. 279 (47), 49281-49288. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUNX1T1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RUNX1T1 antibody can be used for detection of RUNX1T1 by ELISA at 1:312500. RUNX1T1 antibody can be used for detection of RUNX1T1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RUNX1T1 |
|---|---|
| Alternative Names | RUNX1T1 CDR ETO MTG8 AML1T1 ZMYND2 CBFA2T1 |
| Molecular Weight(MW) | 62 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in WB on Human 721_B at 1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | RUNX1T1 antibody can be used for detection of RUNX1T1 by ELISA at 1:312500. RUNX1T1 antibody can be used for detection of RUNX1T1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RUNX1T1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
