Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ETS2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
ETS2 antibody immunoprecipitates ETS2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 μg of ETS2 antibody 7.5% SDS-PAGE The immunoprecipitated ETS2 protein was detected by ETS2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
ETS2 antibody detects ETS2 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. ETS2 antibody dilution: 1:500.Application
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PTHrP promoter.Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ETS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ETS2 (v-ets erythroblastosis virus E26 oncogene homolog 2 (avian))ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ETS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, BovineMouse (87%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ETS2IT1 antibody ETS2 antibody oncogene ETS-2 antibody v-ets avian erythroblastosis virus E2 oncogene homolog 2 antibody v-ets avian erythroblastosis virus E26 oncogene homolog 2 antibody protein C-ets-2 antibody v-ets erythroblastosis virus E26 onco |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ETS2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
ETS2 antibody immunoprecipitates ETS2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ETS2 protein by 2 μg of ETS2 antibody 7.5% SDS-PAGE The immunoprecipitated ETS2 protein was detected by ETS2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
ETS2 antibody detects ETS2 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. ETS2 antibody dilution: 1:500.
Application
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ETS2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PTHrP promoter.
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
