Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EXOSC5 antibody at 1:500 dilution.Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EXOSC5 antibody diluted at 1:1000.Application
EXOSC5 antibody detects EXOSC5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EXOSC5 protein stained by EXOSC5 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | EXOSC5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EXOSC5 (exosome component 5)Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. Required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity. |
| Immunogen | Full length human EXOSC5 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, PigMouse (88%), Rat (88%), Pig (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC111224 antibody MGC12901 antibody RRP41B antibody RRP46 antibody Rrp46p antibody hRrp46p antibody p12B antibody EXOSC5 antibody exosome complex exonuclease RRP46 antibody exosome component Rrp46 antibody chronic myelogenous leukemia tumor anti |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleolus |
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EXOSC5 antibody at 1:500 dilution.
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with EXOSC5 antibody diluted at 1:1000.
Application
EXOSC5 antibody detects EXOSC5 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EXOSC5 protein stained by EXOSC5 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa , HeLa nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
