Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EXOSC8 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
EXOSC8 antibody detects EXOSC8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EXOSC8 antibody diluted by 1:1000.Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000| Product Name | EXOSC8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EXOSC8 (exosome component 8)This gene encodes a 3'-5' exoribonuclease that specifically interacts with mRNAs containing AU-rich elements. The encoded protein is part of the exosome complex that is important for the degradation of numerous RNA species. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EXOSC8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rhesus Monkey, BovineMouse (91%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CIP3 antibody EAP2 antibody OIP2 antibody RP11-421P11.3 antibody RRP43 antibody Rrp43p antibody bA421P11.3 antibody p9 antibody EXOSC8 antibody exosome complex component RRP43 antibody ribosomal RNA-processing protein 43 antibody opa-interacting |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (2) |
| Cellular Localization | Cytoplasm (By similarity) , Nucleus , nucleolus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EXOSC8 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
EXOSC8 antibody detects EXOSC8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EXOSC8 antibody diluted by 1:1000.
Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 , Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
