Application
Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm and membrane in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500.Application
Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Ezrin antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7.5% SDS PAGE diluted at 1:10000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Ezrin antibody at 1:500 dilution.| Product Name | Ezrin antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ezrin (ezrin)The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Rabbit, Chicken, Chimpanzee, BovineXenopus laevis (85%), Dog (98%), Rabbit (94%), Chicken (92%), Chimpanzee (99%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CVIL antibody CVL antibody DKFZp762H157 antibody FLJ26216 antibody MGC1584 antibody VIL2 antibody EZR antibody villin-2 antibody ezrin antibody cytovillin 2 antibody villin 2 (ezrin) antibody p81 antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) (2) |
| Cellular Localization | Apical cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , microvillus membrane; Peripheral membrane protein; Cytoplasmic side , ruffle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cell cortex , cytosk |
Application
Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm and membrane in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500.
Application
Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Ezrin antibody [N2C2], Internal detects Ezrin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Ezrin antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7.5% SDS PAGE diluted at 1:10000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Ezrin antibody at 1:500 dilution.| Positive Control | 293T , A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
