Application
Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using PAR4 (Cleaved-Gly48) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | F2RL3 / PAR-4 (cleaved Gly48) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to F2RL3 / PAR-4 (cleaved Gly48)Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PAR4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PAR4 (cleaved G48) PAR4 (cleaved Gly48) PAR4 cleaved G48 PAR4 cleaved Gly48 Cleaved PAR4 (G48) Cleaved PAR4 (Gly48) |
|---|---|
| Target | PAR4 |
| Molecular Weight(MW) | 33 kDa (note) |
| Tissue Specificity | PAR4 (Cleaved-Gly48) Antibody detects endogenous levels of fragment of activated PAR4 resulting from cleavage adjacent to Gly48. |
Application
Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using PAR4 (Cleaved-Gly48) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
