Application
ELISA analysis with 0.2ug/100ul antigen and serially diluted FABP5 antibody (1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000).Application
Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FABP5 Polyclonal Antibody at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.| Product Name | FABP5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FABP5 |
| Immunogen | KLH conjugated synthetic peptide derived from human Fatty Acid Binding Protein 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-P |
| Recommended Starting Dilutions: For p-ELISA: Use at a dilution of 1:500-1:1000. For IHC-P: Use at a dilution of 1:50-1:500. Optimal working dilutions should be determined experimentally be the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Horse, Pig |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FABP5L1 fatty acid binding protein 5-like 1 (pseudogene) |
|---|---|
| Target | FABP5 |
| Tissue Specificity | FABP5 |
Application
ELISA analysis with 0.2ug/100ul antigen and serially diluted FABP5 antibody (1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000).
Application
Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FABP5 Polyclonal Antibody at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.| Application Notes | Recommended Starting Dilutions: For p-ELISA: Use at a dilution of 1:500-1:1000. For IHC-P: Use at a dilution of 1:50-1:500. Optimal working dilutions should be determined experimentally be the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and less than 0.09% sodium azide. |
