Application
ICC/IF ImageF-Actin antibody [NH3] detects F-Actin protein at cytoskeleton by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: F-Actin protein stained by F-Actin antibody [NH3] diluted at 1:250. Green: NF-M, stained by NF-M antibody diluted at 1:250. Blue: Fluoroshield with DAPI .| Product Name | F-Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [NH3] to F-ActinThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | NH3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-FWB |
| WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-f:1:50~1:100 ELISA:1:5000~1:20000 FACS:1:200~1:500 |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Gene Synonyms | PS1TP5BP1 BRWS1 ACTB |
|---|---|
| Target | F-actin |
| Molecular Weight(MW) | 43 kDa |
| Tissue Specificity | This antibody recognizes human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. |
Application
ICC/IF ImageF-Actin antibody [NH3] detects F-Actin protein at cytoskeleton by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: F-Actin protein stained by F-Actin antibody [NH3] diluted at 1:250. Green: NF-M, stained by NF-M antibody diluted at 1:250. Blue: Fluoroshield with DAPI .| Application Notes | WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC-f:1:50~1:100 ELISA:1:5000~1:20000 FACS:1:200~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
