Application
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Factor IX antibody [N3C3] diluted at 1:500.Application
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Factor IX antibody [N3C3] diluted at 1:250.Application
Factor IX antibody [N3C3] detects secreted Factor IX protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX protein stained by Factor IX antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Factor IX antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Factor IX (coagulation factor IX)This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Factor IX. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Dog, Cat, Pig, Rabbit, Rhesus Monkey, Chimpanzee, BovineMouse (80%), Dog (87%), Cat (83%), Pig (87%), Rabbit (82%), Rhesus Monkey (96%), Chimpanzee (99%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FIX antibody HEMB antibody MGC129641 antibody MGC129642 antibody PTC antibody F9 antibody factor 9 antibody plasma thromboplastin component antibody plasma thromboplastic component antibody coagulation factor IX antibody Christmas factor antibody |
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| Molecular Weight(MW) | 52 kDa (note) |
| Cellular Localization | Secreted |
Application
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Factor IX antibody [N3C3] diluted at 1:500.
Application
Factor IX antibody [N3C3] detects Factor IX protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Factor IX antibody [N3C3] diluted at 1:250.
Application
Factor IX antibody [N3C3] detects secreted Factor IX protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX protein stained by Factor IX antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
