Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human normal gastric epithelium (gland) , using F7 antibody at 1:100 dilution.Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Factor VII antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Factor VII antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Factor VII (coagulation factor VII (serum prothrombin conversion accelerator))This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Factor VII. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | F7 antibody SPCA antibody proconvertin antibody FVII coagulation protein antibody coagulation factor VII antibody eptacog alfa antibody serum prothrombin conversion accelerator antibody coagulation factor VII (serum prothrombin conversion accelerato |
|---|---|
| Target | Factor VII |
| Molecular Weight(MW) | 52 kDa (note) (3) |
| Tissue Specificity | Upon activation of the factor VII, proteolytic cleavage of the peptide bond between Arg(152) and Ile(153) converts Factor VII (FVII) to an activated two-chain form (FVIIa). A heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated. Since the immunogen sequence locates within the heavy chain, it should recognize both Factor VII and Factor VIIa. |
| Cellular Localization | Secreted |
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human normal gastric epithelium (gland) , using F7 antibody at 1:100 dilution.
Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Factor VII antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | human plasma , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
