Mouse monoclonal antibody [RFF-VII/1] to Factor VIIThis gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Immunogen
Purified Factor VII
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RFF-VII/1
Host Species
Mouse
Tested Applications
ELISARIAWB
ELISA: 1/20 - 1/200 Optimal dilution / concentration should be determined by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
SPCA coagulation factor VII (serum prothrombin conversion accelerator) F7
Target
Factor VII
Tissue Specificity
This antibody recognizes Factor VII. It may be used to detect Factor VII in immunoassays in combination with clone RFF-VII/2 as a capture reagent.
Application
Application
ELISA analysis of human serum using Factor VII antibody [RFF-VII/1].
Application Notes
ELISA: 1/20 - 1/200 Optimal dilution / concentration should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.