Application
Immunofluorescence analysis of methanol-fixed HeLa, using Factor X antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Factor X antibody detects F10 protein by western blot analysis. A. 30 µg GL261 whole lysate/extract B. 30 µg C8D30 whole cell lysate/extract 10% SDS-PAGE Factor X antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Factor X antibody detects F10 protein by western blot analysis. A. 30µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human F10-transfected 293T cells 10% SDS-PAGE Factor X antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Factor X antibody at 1:100 dilution.| Product Name | Factor X antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Factor X (coagulation factor X)This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Factor X. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rabbit, Rhesus MonkeyRabbit (81%), Rhesus Monkey (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FX antibody FXA antibody F10 antibody Stuart-Prower factor antibody prothrombinase antibody coagulation factor X antibody factor Xa antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Factor X antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Factor X antibody detects F10 protein by western blot analysis. A. 30 µg GL261 whole lysate/extract B. 30 µg C8D30 whole cell lysate/extract 10% SDS-PAGE Factor X antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Factor X antibody detects F10 protein by western blot analysis. A. 30µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human F10-transfected 293T cells 10% SDS-PAGE Factor X antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Factor X antibody at 1:100 dilution.| Positive Control | HeLa , human F10-transfected 293T cells , GL261 , C8D30 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
