Application
Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using FA13A (Cleaved-Gly39) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | Factor XIIIa (cleaved Gly39) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Factor XIIIa (cleaved Gly39)This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FA13A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FA13A FA13A (cleaved G39) FA13A (cleaved Gly39) FA13A cleaved G39 FA13A cleaved Gly39 Cleaved FA13A (G39) Cleaved FA13A (Gly39) |
|---|---|
| Molecular Weight(MW) | 79 kDa (note) |
| Tissue Specificity | Factor XIIIa (cleaved Gly39) antibody detects endogenous levels of fragment of activated Factor XIIIa resulting from cleavage adjacent to Gly39. |
Application
Western blot analysis of extracts from Jurkat cells, treated with etoposide 25uM 24h, using FA13A (Cleaved-Gly39) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
