Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FADD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE FADD antibody diluted at 1:1000Application
FADD antibody [N3C2], Internal detects FADD protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. FADD antibody [N3C2], Internal dilution: 1:250.| Product Name | FADD antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FADD (Fas (TNFRSF6)-associated via death domain)The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human FADD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC8528 antibody MORT1 antibody FADD antibody mediator of receptor induced toxicity antibody protein FADD antibody Fas-associating protein with death domain antibody growth-inhibiting gene 3 protein antibody mediator of receptor-induced toxicity ant |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FADD antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE FADD antibody diluted at 1:1000
Application
FADD antibody [N3C2], Internal detects FADD protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. FADD antibody [N3C2], Internal dilution: 1:250.| Positive Control | A549 , K562 , THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
