Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FADS1 antibody at 1:250 dilution.Application
FADS1 antibody detects FADS1 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FADS1 protein stained by FADS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | FADS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FADS1 (fatty acid desaturase 1)The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human FADS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, PigMouse (88%), Rat (87%), Pig (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D5D antibody FADS6 antibody FADSD5 antibody FLJ38956 antibody FLJ90273 antibody LLCDL1 antibody TU12 antibody FADS1 antibody linoleoyl-CoA desaturase (delta-6-desaturase)-like 1 antibody delta-5 desaturase antibody delta-5 fatty acid desaturase a |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein |
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FADS1 antibody at 1:250 dilution.
Application
FADS1 antibody detects FADS1 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FADS1 protein stained by FADS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
