Application
Untreated (–) and treated (+) HUVEC whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody diluted at 1:500.Application
Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody diluted at 1:500.| Product Name | FAK (phospho Tyr397) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FAK (phospho Tyr397) (protein tyrosine kinase 2)This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human FAK (phospho Tyr397). The exact sequence is proprietary. |
| Modification | p-Tyr397 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (100%), Rat (100%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | FAK (phospho Tyr397) |
|---|---|
| Molecular Weight(MW) | 119 kDa (note) (3) |
| Cellular Localization | Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Untreated (–) and treated (+) HUVEC whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody diluted at 1:500.
Application
Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody diluted at 1:500.| Positive Control | HUVEC , HUVEC (5 ng/ml VEGF treatment for 10 min) , HepG2 (200 ng/ml EGF treatment for 30 min) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
