Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | FAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FAK (PTK2 protein tyrosine kinase 2)This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human FAK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Chicken, BovineMouse (85%), Chicken (86%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FADK antibody FAK antibody FAK1 antibody FRNK antibody pp125FAK antibody PTK2 antibody FADK 1 antibody FAK-related non-kinase polypeptide antibody protein phosphatase 1 regulatory subunit 71 antibody focal adhesion kinase 1 antibody PTK2 protein |
|---|---|
| Molecular Weight(MW) | 119 kDa (note) (3) |
| Cellular Localization | Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
