Application
Lane 1: Rat Liver Tissue Lysate Lane 2: 293T Cell Lysate Lane 3: HELA Cell Lysate| Product Name | MYPT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MYPT1Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Seve |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminal of human MYPT1, identical to the related rat and mouse sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:250~1:1000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | protein phosphatase 1 regulatory subunit 12A MYPT1 M130 MBS |
|---|---|
| Target | MYPT1 |
Application
Lane 1: Rat Liver Tissue Lysate Lane 2: 293T Cell Lysate Lane 3: HELA Cell Lysate| Application Notes | WB:1:250~1:1000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 5% BSA, 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% NaN3. |
