Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
FAM120A antibody detects FAM120A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAM120A protein stained by FAM120A antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using FAM120A antibody at 1:250 dilution.| Product Name | FAM120A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FAM120A (family with sequence similarity 120A)May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FAM120A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (95%), Rat (93%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C9orf10 antibody DNAPTP1 antibody DNAPTP5 antibody MGC111527 antibody MGC133257 antibody OSSA antibody FAM120A antibody oxidative stress-associated Src activator antibody DNA polymerase-transactivated protein 5 antibody constitutive coactivator of |
|---|---|
| Molecular Weight(MW) | 122 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Cell membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
FAM120A antibody detects FAM120A protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAM120A protein stained by FAM120A antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using FAM120A antibody at 1:250 dilution.| Positive Control | MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
