Application
Immunohistochemical analysis of paraffin-embedded human T(gastric cancer) , using NSMAF antibody at 1:100 dilution.Application
Western Blot analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate. B: NSMAF transfected lysate. 7.5% SDS PAGE diluted at 1:500| Product Name | FAN antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to FAN (neutral sphingomyelinase (N-SMase) activation associated factor)This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FAN. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (93%), Rat (91%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FAN antibody NSMAF antibody protein FAN antibody factor associated with N-SMase activation antibody factor associated with neutral sphingomyelinase activation antibody neutral sphingomyelinase (N-SMase) activation associated factor antibody |
|---|---|
| Target | FAN |
| Molecular Weight(MW) | 104 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded human T(gastric cancer) , using NSMAF antibody at 1:100 dilution.
Application
Western Blot analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody. A: Non-transfected lysate. B: NSMAF transfected lysate. 7.5% SDS PAGE diluted at 1:500| Positive Control | FAN tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
