Application
Western blot using GeneTex's affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye™800 fluorescence images were captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.| Product Name | FANCA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FANCAFANCA is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. |
| Immunogen | Synthetic peptide: SRSYDHSENSDLVFG-C conjugated to KLH, corresponding to amino acids 995 - 1009 of Human FANCA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanChimpanzee |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FA FAA FANCH FA H FACA FA1 FAH |
|---|---|
| Target | FANCA |
| Cellular Localization | Nuclear (major) and cytoplasmic (minor) |
Application
Western blot using GeneTex's affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye™800 fluorescence images were captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
