Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE FANCJ antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500.Application
FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | FANCJ antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FANCJ (BRCA1 interacting protein C-terminal helicase 1)The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human FANCJ. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BACH1 antibody FANCJ antibody FLJ90232 antibody MGC126521 antibody MGC126523 antibody OF antibody BRIP1 antibody Fanconi anemia group J protein antibody BRCA1/BRCA2-associated helicase 1 antibody BRCA1-binding helicase-like protein BACH1 antibody |
|---|---|
| Molecular Weight(MW) | 141 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE FANCJ antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500.
Application
FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
