Application
WB analysis of (1) HeLa and (2) Jurkat lysates using FAS antibody [4F8D6].Application
WB analysis of human FAS (a.a. 87-278) recombinant protein using FAS antibody [4F8D6].Application
FACS analysis of HeLa cells using FAS antibody [4F8D6] (green) or a negative control (red).Application
ELISA analysis of control antigen and antigen using FAS antibody [4F8D6].| Product Name | Fas antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4F8D6] to FasThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the tra |
| Immunogen | The immunogen corresponding to human FAS expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4F8D6 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | APT1 FASTM FAS1 ALPS1A APO-1 TNFRSF6 CD95 |
|---|---|
| Target | Fas |
| Molecular Weight(MW) | 37.7 kDa (note) |
Application
WB analysis of (1) HeLa and (2) Jurkat lysates using FAS antibody [4F8D6].
Application
WB analysis of human FAS (a.a. 87-278) recombinant protein using FAS antibody [4F8D6].
Application
FACS analysis of HeLa cells using FAS antibody [4F8D6] (green) or a negative control (red).
Application
ELISA analysis of control antigen and antigen using FAS antibody [4F8D6].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
