For FACS: Use at a dilution of 1:150-1:300. For IHC-Fr: Use at a dilution of 1:50-1:100. For IHC-P: Use at a dilution of 1:50-1:100. Optimal dilutions/concentrations should be determined by the researcher.:
Reacts with CD95 a 40-45kD cell surface glycoprotein containing 8kD of N-glycosidic-linked polysaccharide and that is expressed by the CD45RAlow and CD45ROhigh subset of memory T cells.
Positive Control
Tonsil, Spleen, Thymus
Application Notes
For FACS: Use at a dilution of 1:150-1:300. For IHC-Fr: Use at a dilution of 1:50-1:100. For IHC-P: Use at a dilution of 1:50-1:100. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer
20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.