| Product Name | Fas antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [DX2] to FasMany cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. One of the most studied receptors include the Fas receptor. Apoptosis mediated by both signaling cascades results in activation of a family of cystein proteases known as caspases. Engagement of Fas by its ligand, or by an appropriate antibody, results in the rapid induction of programmed cell death in susceptible cell lines. This process bypasses the usual long sequence of signaling enzymes and immediately activates preexisting caspases. |
| Immunogen | Mouse L cells transfected with a human Fas/CD95 cDNA. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DX2 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| FACS: Use 10μl for 10^6 cells 1 ug per 100 ul blood. WB: Use at an assay dependent dilution. Predicted molecular weight: 45-48 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CD95 FAS1 APO1 Apo-1 Fas Apo1 Fas 355 APT1 FASTM P25445 134637 ALPS1A APO-1 FAS TNFRSF6 APO 1 Apo 1 Fas |
|---|---|
| Target | Fas |
| Cellular Localization | Plasma membrane (isoform 1) and Secreted (isoforms 2-6). |
| Positive Control | Burkitt's lymphoma Raji cells (FACS) |
|---|---|
| Application Notes | FACS: Use 10μl for 10^6 cells 1 ug per 100 ul blood. WB: Use at an assay dependent dilution. Predicted molecular weight: 45-48 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20 C |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS |
